Figure 4

Inhibition of PGC1α gene upregulation by FAs. Primary hepatocytes were cultured and pre-treated with FAs mixture (2 : 1 oleate:palmitate with 1% BSA) or with FAs-Br mixture (2 : 1 oleate:2-Bromopalmitate with 1% BSA) to final concentration of 1 mM FAs for the indicated times. After pre-treatments, cells were treated with the gluconeogenic inducers glucagon and cAMP (A) or dexamethasone and cAMP (B) for 6 hours. mRNA expression levels of PGC1α were measured by quantitative real-time RT-PCR. Data are expressed as means ± SD, presented relatively to basal expression level (of cells not pre-treated with FAs nor treated with gluconeogenic inducers), which was normalized to 1. n = 3; Comparisons were performed using Tukey’s honestly significant differences (HSD) test. *P < .05 vs basal expression level; †P < .05 vs respective control treatment (of cells not treated with gluconeogenic inducers); ‡P < .05 vs cells pre-treated for 1 hour with FAs and then treated with gluconeogenic inducers; §P < .05 vs cells pre-treated for 18 hours with FAs-Br and then treated with gluconeogenic inducers.