Figure 5

Inhibition of CREB activation by FAs. Primary hepatocytes were cultured and pre-treated with FAs mixture (2 : 1 oleate:palmitate with 1% BSA) to final concentration of 1 mM FAs for the indicated times. After pre-treatments, cells were treated with the gluconeogenic inducers glucagon and cAMP (A) or dexamethasone and cAMP (B) for 6 hours. CREB phosphorylation levels were measured by western-blot analysis. Quantification of bands was performed by scanning densitometry. The bars represent the means ± SD of the values, relatively to the basal expression level (of cells not pre-treated with FAs nor treated with gluconeogenic inducers) average value, which was normalized to 100. n = 3; Comparisons were performed using Tukey’s honestly significant differences (HSD) test. Representative bands are shown above bar graphs. *P < .05 vs basal expression level; ‡P < .05 vs cells pre-treated for 1 hour with FAs and then treated with gluconeogenic inducers.