Table 1 Primer- or probe- sequences for real-time quantitative PCR
Transcript | primer/Probe sequence (5′ to 3′) | |
|---|---|---|
DGAT | F | TGTCTGAGCGAACAAAGAATCTTG |
R | CGTGGGCGAACGGCTACT | |
FAM-ACGATGGCACCTCAGA-MGB | ||
DGAT2 | F | GTCTTGGAGGGCTGAGAGGAT |
R | AAGAATAAAGGATCTGCCCTGTCA | |
FAM-CCAGTGCCCCATCG-MGB | ||
Beta-Actin | F | GCTCTGGCTCCTAGACCAT |
R | GCCACCGATCCACACACAGT | |
FAM-ATCAAGATCATTGCTCCTC-MGB | ||
18SrRNA | F | CGGCTACCACATCCAAGGAA |
R | GCTGGAATTACCGCGGCT | |
FAM-TGCTGGCACCAGACTTGCCCTC-MGB | ||
GAPDH | F | CGTCGCGAAGGATACTCT |
R | GGCAAGCAGATTTCACTGTGAAG | |
FAM-TCGTCAACGGCCACCG-MGB | ||