Figure 3

ATRA and TO-901317 synergistically induce ABCA1 expression. A) Primed human CD4+ T cells were incubated with 1 μM of ATRA and 1 μM of LXR-agonist TO-901317 for 2 days. ABCA1 mRNA was measured and normalized to the level of GAPDH mRNA and relative RNA fold changes compared to that from DMSO treated cells were plotted. Results are representative of three independent experiments. Data are presented as mean ± the standard deviation. B) ABCA1 and GAPDH proteins were detected as described in Methods. C) The intensity of the ABCA1 protein bands in (B) was quantified and normalized to that of GAPDH. The relative intensity compared to DMSO treatment was plotted. Results are representative of three independent experiments. Data are presented as mean ± the standard deviation. D) and E) The synergistic effects of 5 μM of ATRA and 1 μM of TO-901317 on ABCA1 RNA and protein expression, respectively, in Jurkat cells. F) The intensity of the bands representing ABCA1 protein in (E) was quantified and normalized to that of GAPDH protein. The relative intensity compared to DMSO treatment was plotted.