|
NO
|
Assay mixture
|
Resveratrol
|
Pterostilbene
|
Morin Hydrate
|
Nicotinic acid
|
Quercetin*
|
|---|
|
Average digital values of relative luminescence units (RLU)
|
|---|
|
1
|
Medium + cells = A
|
87458
|
87458
|
87458
|
87458
|
87458
|
|
2
|
A + DMSO control2
|
95222 ± 2014 (100)3
|
95222 ± 2014(100)3
|
95750 ± 2014 (100)3
|
95750 ± 2014 (100)3
|
95750 ± 2014 (100)3
|
|
3
|
10 μM
|
7376 ± 107 (8)
|
27543 ± 947 (29)
|
89557 ± 1958 (94)
|
95240 ± 2020 (99)
|
61823 ± 2206 (65)
|
|
4
|
20 μM
|
6996 ± 143 (7)
|
24177 ± 1775 (25)
|
67338 ± 1626 (71)
|
95342 ± 1896 (100)
|
36772 ± 1201 (39)
|
|
5
|
40 μM
|
2742 ± 138 (3)
|
23574 ± 1126 (25)
|
55078 ± 1444 (58)
|
94232 ± 1962 (98)
|
19805 ± 732 (21)
|
|
6
|
80 μM
|
2261 ± 124 (2)
|
21639 ± 1667 (23)
|
41178 ± 1099 (43)
|
94155 ± 1926 (98)
|
17478 ± 783 (18)
|
|
7
|
160 μM
|
1688 ± 121 (2)
|
19483 ± 1207 (20)
|
33814 ± 134 (36)
|
95165 ± 1966 (99)
|
13827 ± 621 (15)
|
|
8
|
320 μM
|
1590 ± 92 (2)
|
13380 ± 1341 (14)
|
27448 ± 88 (29)
|
94124 ± 2038 (98)
|
5896 ± 321 (6)
|
- 1 Chymotrypsin-like activity was quantitated by measuring luminescence after treating RAW 264.7 whole cells (1x104) with various concentrations (10 μM - 320 μM) of resveratrol, pterostilbene, morin hydrate, nicotinic acid, or quercetin as described in Methods. The relative luminescence units (RLU) of assays were read in a Promega Plate Luminometer.
- 2Medium + RAW 264.7 whole cells + 0.2 % dimethyl sulfioxide (DMSO) was used as control. 3Percentages based on digital values of RLU compared to control are in parenthesis. *Similar results were reported in Lipids in Health and Disease 2011, 10:177.
