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Table 1 Resveratrol, pterostilbene, morin hydrate, and quercetin inhibit chymotrypsin-like proteasomal activity 1

From: Inhibition of nitric oxide and inflammatory cytokines in LPS-stimulated murine macrophages by resveratrol, a potent proteasome inhibitor

NO Assay mixture Resveratrol Pterostilbene Morin Hydrate Nicotinic acid Quercetin*
Average digital values of relative luminescence units (RLU)
1 Medium + cells = A 87458 87458 87458 87458 87458
2 A + DMSO control2 95222 ± 2014 (100)3 95222 ± 2014(100)3 95750 ± 2014 (100)3 95750 ± 2014 (100)3 95750 ± 2014 (100)3
3 10 μM 7376 ± 107 (8) 27543 ± 947 (29) 89557 ± 1958 (94) 95240 ± 2020 (99) 61823 ± 2206 (65)
4 20 μM 6996 ± 143 (7) 24177 ± 1775 (25) 67338 ± 1626 (71) 95342 ± 1896 (100) 36772 ± 1201 (39)
5 40 μM 2742 ± 138 (3) 23574 ± 1126 (25) 55078 ± 1444 (58) 94232 ± 1962 (98) 19805 ± 732 (21)
6 80 μM 2261 ± 124 (2) 21639 ± 1667 (23) 41178 ± 1099 (43) 94155 ± 1926 (98) 17478 ± 783 (18)
7 160 μM 1688 ± 121 (2) 19483 ± 1207 (20) 33814 ± 134 (36) 95165 ± 1966 (99) 13827 ± 621 (15)
8 320 μM 1590 ± 92 (2) 13380 ± 1341 (14) 27448 ± 88 (29) 94124 ± 2038 (98) 5896 ± 321 (6)
  1. 1 Chymotrypsin-like activity was quantitated by measuring luminescence after treating RAW 264.7 whole cells (1x104) with various concentrations (10 μM - 320 μM) of resveratrol, pterostilbene, morin hydrate, nicotinic acid, or quercetin as described in Methods. The relative luminescence units (RLU) of assays were read in a Promega Plate Luminometer.
  2. 2Medium + RAW 264.7 whole cells + 0.2 % dimethyl sulfioxide (DMSO) was used as control. 3Percentages based on digital values of RLU compared to control are in parenthesis. *Similar results were reported in Lipids in Health and Disease 2011, 10:177.