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Table 1 Resveratrol, pterostilbene, morin hydrate, and quercetin inhibit chymotrypsin-like proteasomal activity 1

From: Inhibition of nitric oxide and inflammatory cytokines in LPS-stimulated murine macrophages by resveratrol, a potent proteasome inhibitor

NO

Assay mixture

Resveratrol

Pterostilbene

Morin Hydrate

Nicotinic acid

Quercetin*

Average digital values of relative luminescence units (RLU)

1

Medium + cells = A

87458

87458

87458

87458

87458

2

A + DMSO control2

95222 ± 2014 (100)3

95222 ± 2014(100)3

95750 ± 2014 (100)3

95750 ± 2014 (100)3

95750 ± 2014 (100)3

3

10 μM

7376 ± 107 (8)

27543 ± 947 (29)

89557 ± 1958 (94)

95240 ± 2020 (99)

61823 ± 2206 (65)

4

20 μM

6996 ± 143 (7)

24177 ± 1775 (25)

67338 ± 1626 (71)

95342 ± 1896 (100)

36772 ± 1201 (39)

5

40 μM

2742 ± 138 (3)

23574 ± 1126 (25)

55078 ± 1444 (58)

94232 ± 1962 (98)

19805 ± 732 (21)

6

80 μM

2261 ± 124 (2)

21639 ± 1667 (23)

41178 ± 1099 (43)

94155 ± 1926 (98)

17478 ± 783 (18)

7

160 μM

1688 ± 121 (2)

19483 ± 1207 (20)

33814 ± 134 (36)

95165 ± 1966 (99)

13827 ± 621 (15)

8

320 μM

1590 ± 92 (2)

13380 ± 1341 (14)

27448 ± 88 (29)

94124 ± 2038 (98)

5896 ± 321 (6)

  1. 1 Chymotrypsin-like activity was quantitated by measuring luminescence after treating RAW 264.7 whole cells (1x104) with various concentrations (10 μM - 320 μM) of resveratrol, pterostilbene, morin hydrate, nicotinic acid, or quercetin as described in Methods. The relative luminescence units (RLU) of assays were read in a Promega Plate Luminometer.
  2. 2Medium + RAW 264.7 whole cells + 0.2 % dimethyl sulfioxide (DMSO) was used as control. 3Percentages based on digital values of RLU compared to control are in parenthesis. *Similar results were reported in Lipids in Health and Disease 2011, 10:177.
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Lipids in Health and Disease

ISSN: 1476-511X