|
NO
|
Treatments
|
RT-PCR data (*Ratios of optical density of gene expression of cytokines/β-actin).
|
|---|
|
TNF-α
|
IL-1β
|
IL-6
|
iNOS
|
|---|
|
1
|
Media + Cells = A
|
0.05
|
0.05
|
0.1
|
0.2
|
|
2
|
A + LPS (10 ng/well) = B
|
0.83
|
1.35
|
0.93
|
1.12
|
|
3
|
B + 0.2 % DMSO = C
|
0.80 ± 0.03a (100)2
|
1.32 ± 0.03a (100)2
|
0.96 ± 0.03a (100)2
|
1.04 ± 0.02a (100)2
|
|
4
|
C + Resveratrol (16.0 μM)
|
0.18 ± 0.02e (23)
|
0.51 ± 0.03e (39)
|
0.21 ± 0.0e (22)
|
0.65 ± 0.02c (63)
|
|
5
|
C + Pterostilbene (16.0 μM)
|
0.38 ± 0.03d (48)
|
0.75 ± 0.03d (57)
|
0.62 ± 0.03c (65)
|
0.92 ± 0.03b (88)
|
|
6
|
C + Morin hydrate (16.0 μM)
|
0.55 ± 0.03b (69)
|
0.92 ± 0.04c (70)
|
0.63 ± 0.02c (66)
|
0.53 ± 0.03d (51)
|
|
7
|
C + Nicotinic acid (16.0 μM)
|
0.76 ± 0.05a (95)
|
1.12 ± 0.02b (85)
|
0.75 ± 0.03b (78)
|
0.66 ± 0.05c (64)
|
|
8
|
C + Quercetin (16.0 μM)
|
0.45 ± 0.02c (56)
|
0.22 ± 0.02f (17)
|
0.25 ± 0.0d (26)
|
0.37 ± 0.03e (36)
|
- 1RAW 264.7 cells were treated with resveratrol, pterostilbene, morin hydrate, nicotinic acid, or quercetin (16 μM) for 1 h, followed by treatment with LPS (10 ng/well) for 4 h. Total RNA was extracted, reverse transcribed, and the resulting DNA was amplified and analyzed by real time PCR to quantitate expression of TNF-α, IL-1β, IL-6, and iNOS genes [5, 21].
- Cell viability was >95% in all treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
- 2Percentages of *digital values of relative optical density of each cytokine/β-actin of each treatment are in parenthesis. a-fValues in a column not sharing a common superscript letter are significantly different at P < 0.05.
