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Table 2 Resveratrol, pterostilbene, morin hydrate, nicotinic acid, and quercetin inhibit expression of TNF-α, IL-1β, IL-6, and iNOS genes in LPS-stimulated RAW 264.7 cells 1

From: Inhibition of nitric oxide and inflammatory cytokines in LPS-stimulated murine macrophages by resveratrol, a potent proteasome inhibitor

NO Treatments RT-PCR data (*Ratios of optical density of gene expression of cytokines/β-actin).
TNF-α IL-1β IL-6 iNOS
1 Media + Cells = A 0.05 0.05 0.1 0.2
2 A + LPS (10 ng/well) = B 0.83 1.35 0.93 1.12
3 B + 0.2 % DMSO = C 0.80 ± 0.03a (100)2 1.32 ± 0.03a (100)2 0.96 ± 0.03a (100)2 1.04 ± 0.02a (100)2
4 C + Resveratrol (16.0 μM) 0.18 ± 0.02e (23) 0.51 ± 0.03e (39) 0.21 ± 0.0e (22) 0.65 ± 0.02c (63)
5 C + Pterostilbene (16.0 μM) 0.38 ± 0.03d (48) 0.75 ± 0.03d (57) 0.62 ± 0.03c (65) 0.92 ± 0.03b (88)
6 C + Morin hydrate (16.0 μM) 0.55 ± 0.03b (69) 0.92 ± 0.04c (70) 0.63 ± 0.02c (66) 0.53 ± 0.03d (51)
7 C + Nicotinic acid (16.0 μM) 0.76 ± 0.05a (95) 1.12 ± 0.02b (85) 0.75 ± 0.03b (78) 0.66 ± 0.05c (64)
8 C + Quercetin (16.0 μM) 0.45 ± 0.02c (56) 0.22 ± 0.02f (17) 0.25 ± 0.0d (26) 0.37 ± 0.03e (36)
  1. 1RAW 264.7 cells were treated with resveratrol, pterostilbene, morin hydrate, nicotinic acid, or quercetin (16 μM) for 1 h, followed by treatment with LPS (10 ng/well) for 4 h. Total RNA was extracted, reverse transcribed, and the resulting DNA was amplified and analyzed by real time PCR to quantitate expression of TNF-α, IL-1β, IL-6, and iNOS genes [5, 21].
  2. Cell viability was >95% in all treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
  3. 2Percentages of *digital values of relative optical density of each cytokine/β-actin of each treatment are in parenthesis. a-fValues in a column not sharing a common superscript letter are significantly different at P < 0.05.