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Table 3 Resveratrol, pterostilbene, morin hydrate, nicotinic acid, and quercetin inhibit expression of TNF-α, IL-1β, IL-6, and iNOS genes in peritoneal macrophages from C57BL/6 mice 1

From: Inhibition of nitric oxide and inflammatory cytokines in LPS-stimulated murine macrophages by resveratrol, a potent proteasome inhibitor

NO

Treatments

RT-PCR data (Ratios of digital values of optical density of gene expression of cytokines/β-actin).

TNF-α

IL-1β

IL-6

iNOS

1

Media + Cells = A

0.05

0.05

0.1

0.2

2

A + LPS (10 ng/well) = B

0.45

5.32

8.67

4.67

3

B + 0.4 % DMSO = C

0.36 ± 0.02a (100)2

6.89 ± 2.01a (100)2

12.34 ± 1.96a (100)2

5.67 ± 1.90a (100)2

4

C + Resveratrol (40.0 μM)

0.10 ± 0.01c (28)

1.39 ± 0.04c (20)

2.16 ± 0.60c (18)

3.93 ± 1.50b (69)

5

C + Pterostilbene (40.0 μM)

0.11 ± 0.01c (31)

1.74 ± 0.48b (25)

2.33 ± 0.82c (19)

3.76 ± 1.40b,c (66)

6

C + Morin hydrate (40.0 μM)

0.14 ± 0.05b (39)

1.87 ± 0.49b (27)

3.03 ± 1.03b (25)

3.47 ± 0.03b,c (61)

7

C + Nicotinic acid (40.0 μM)

0.33 ± 0.03a (92)

6.27 ± 0.42a (91)

10.86 ± 0.91a (88)

4.81 ± 0.66a (85)

8

C + Quercetin (40.0 μM)

0.11 ± 0.04c (31)

2.14 ± 0.46b (31)

3.24 ± 0.93b (26)

3.65 ± 1.18b,c (64)

  1. 1Thioglycolate-elicited peritoneal macrophages from 8-week-old C57BL/6 mice were treated with resveratrol, pterostilbene, morin hydrate, Cell viability was >95% in all the treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
  2. Cell viability was >95 % in all the treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
  3. 2Percentages of digital values of relative optical density of each cytokine/β-actin of each treatment are in parenthesis.
  4. a-cValues in a column not sharing a common superscript letter are significantly different at P < 0.05.