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Table 3 Resveratrol, pterostilbene, morin hydrate, nicotinic acid, and quercetin inhibit expression of TNF-α, IL-1β, IL-6, and iNOS genes in peritoneal macrophages from C57BL/6 mice 1

From: Inhibition of nitric oxide and inflammatory cytokines in LPS-stimulated murine macrophages by resveratrol, a potent proteasome inhibitor

NO Treatments RT-PCR data (Ratios of digital values of optical density of gene expression of cytokines/β-actin).
TNF-α IL-1β IL-6 iNOS
1 Media + Cells = A 0.05 0.05 0.1 0.2
2 A + LPS (10 ng/well) = B 0.45 5.32 8.67 4.67
3 B + 0.4 % DMSO = C 0.36 ± 0.02a (100)2 6.89 ± 2.01a (100)2 12.34 ± 1.96a (100)2 5.67 ± 1.90a (100)2
4 C + Resveratrol (40.0 μM) 0.10 ± 0.01c (28) 1.39 ± 0.04c (20) 2.16 ± 0.60c (18) 3.93 ± 1.50b (69)
5 C + Pterostilbene (40.0 μM) 0.11 ± 0.01c (31) 1.74 ± 0.48b (25) 2.33 ± 0.82c (19) 3.76 ± 1.40b,c (66)
6 C + Morin hydrate (40.0 μM) 0.14 ± 0.05b (39) 1.87 ± 0.49b (27) 3.03 ± 1.03b (25) 3.47 ± 0.03b,c (61)
7 C + Nicotinic acid (40.0 μM) 0.33 ± 0.03a (92) 6.27 ± 0.42a (91) 10.86 ± 0.91a (88) 4.81 ± 0.66a (85)
8 C + Quercetin (40.0 μM) 0.11 ± 0.04c (31) 2.14 ± 0.46b (31) 3.24 ± 0.93b (26) 3.65 ± 1.18b,c (64)
  1. 1Thioglycolate-elicited peritoneal macrophages from 8-week-old C57BL/6 mice were treated with resveratrol, pterostilbene, morin hydrate, Cell viability was >95% in all the treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
  2. Cell viability was >95 % in all the treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
  3. 2Percentages of digital values of relative optical density of each cytokine/β-actin of each treatment are in parenthesis.
  4. a-cValues in a column not sharing a common superscript letter are significantly different at P < 0.05.