|
NO
|
Treatments
|
RT-PCR data (Ratios of digital values of optical density of gene expression of cytokines/β-actin).
|
|---|
|
TNF-α
|
IL-1β
|
IL-6
|
iNOS
|
|---|
|
1
|
Media + Cells = A
|
0.05
|
0.05
|
0.1
|
0.2
|
|
2
|
A + LPS (10 ng/well) = B
|
0.62
|
8.27
|
14.45
|
7.76
|
|
3
|
B + 0.4 % DMSO = C
|
0.74 ± 0.11a (100)2
|
10.68 ± 1.18a (100)2
|
15.68 ± 1.13a (100)2
|
9.85 ± 1.04a (100)2
|
|
4
|
C + Resveratrol (40.0 μM)
|
0.28 ± 0.06c (38)
|
3.25 ± 0.97b (30)
|
4.43 ± 1.23b,c (28)
|
7.18 ± 0.88b (73)
|
|
5
|
C + Pterostilbene (40.0 μM)
|
0.35 ± 0.08b (47)
|
3.55 ± 1.00b (33)
|
5.08 ± 0.76b,c (32)
|
6.68 ± 1.10b (68)
|
|
6
|
C + Morin hydrate (40.0 μM)
|
0.32 ± 0.09c (43)
|
3.75 ± 1.04b (35)
|
5.68 ± 1.09b (36)
|
7.28 ± 0.97b (74)
|
|
7
|
C + Nicotinic acid (40.0 μM)
|
0.65 ± 0.11a (88)
|
9.74 ± 1.33a (91)
|
12.76 ± 0.91a (81)
|
8.17 ± 1.05a (83)
|
|
8
|
C + Quercetin (40.0 μM)
|
0.30 ± 0.12c (41)
|
3.63 ± 1.40b (34)
|
6.10 ± 0.77b (39)
|
7.12 ± 1.07b (72)
|
- 1Thioglycolate-elicited peritoneal macrophages from 8-week-old BALB/c mice were treated with resveratrol, pterostilbene, morin hydrate, nicotinic acid, or quercetin (40 μM) for 1 h, followed by treatment with LPS (10 ng/well) for 4 h. Total RNA was extracted, reverse transcribed, and the resulting DNA was amplified and analyzed by real time PCR to quantitate expression of TNF-α, IL-1β, IL-6, and iNOS genes [5, 21]. Cell viability was >95 % in all the treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
- 2Percentages of digital values of relative optical density of each cytokine/β-actin of each treatment are in parenthesis.
- a-cValues in a column not sharing a common superscript letter are significantly different at P < 0.05.
