Skip to main content

Table 4 Resveratrol, pterostilbene, morin hydrate, nicotinic acid, and quercetin inhibit expression of TNF-α, IL-1β, IL-6, and iNOS genes in peritoneal macrophages from BALB/c mice 1

From: Inhibition of nitric oxide and inflammatory cytokines in LPS-stimulated murine macrophages by resveratrol, a potent proteasome inhibitor

NO Treatments RT-PCR data (Ratios of digital values of optical density of gene expression of cytokines/β-actin).
TNF-α IL-1β IL-6 iNOS
1 Media + Cells = A 0.05 0.05 0.1 0.2
2 A + LPS (10 ng/well) = B 0.62 8.27 14.45 7.76
3 B + 0.4 % DMSO = C 0.74 ± 0.11a (100)2 10.68 ± 1.18a (100)2 15.68 ± 1.13a (100)2 9.85 ± 1.04a (100)2
4 C + Resveratrol (40.0 μM) 0.28 ± 0.06c (38) 3.25 ± 0.97b (30) 4.43 ± 1.23b,c (28) 7.18 ± 0.88b (73)
5 C + Pterostilbene (40.0 μM) 0.35 ± 0.08b (47) 3.55 ± 1.00b (33) 5.08 ± 0.76b,c (32) 6.68 ± 1.10b (68)
6 C + Morin hydrate (40.0 μM) 0.32 ± 0.09c (43) 3.75 ± 1.04b (35) 5.68 ± 1.09b (36) 7.28 ± 0.97b (74)
7 C + Nicotinic acid (40.0 μM) 0.65 ± 0.11a (88) 9.74 ± 1.33a (91) 12.76 ± 0.91a (81) 8.17 ± 1.05a (83)
8 C + Quercetin (40.0 μM) 0.30 ± 0.12c (41) 3.63 ± 1.40b (34) 6.10 ± 0.77b (39) 7.12 ± 1.07b (72)
  1. 1Thioglycolate-elicited peritoneal macrophages from 8-week-old BALB/c mice were treated with resveratrol, pterostilbene, morin hydrate, nicotinic acid, or quercetin (40 μM) for 1 h, followed by treatment with LPS (10 ng/well) for 4 h. Total RNA was extracted, reverse transcribed, and the resulting DNA was amplified and analyzed by real time PCR to quantitate expression of TNF-α, IL-1β, IL-6, and iNOS genes [5, 21]. Cell viability was >95 % in all the treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
  2. 2Percentages of digital values of relative optical density of each cytokine/β-actin of each treatment are in parenthesis.
  3. a-cValues in a column not sharing a common superscript letter are significantly different at P < 0.05.