|
NO
|
Treatments
|
RT-PCR data (Ratios of digital values of optical density of gene expression of cytokines/β-actin).
|
|---|
|
TNF-α
|
IL-1β
|
IL-6
|
iNOS
|
|---|
|
RAW 264.7 cells.
|
|
1
|
Media + Cells = A
|
0.05
|
0.05
|
0.1
|
0.2
|
|
2
|
A + LPS (10 ng/well) = B
|
0.83
|
1.35
|
0.98
|
1.12
|
|
3
|
B + 0.2 % DMSO = C
|
0.80 ± 0.03a (100)2
|
1.32 ± 0.03a (100)2
|
0.96 ± 0.03a (100)2
|
1.04 ± 0.02a (100)2
|
|
4
|
C + δ-Tocotrienol (16.0 μM)
|
0.32 ± 0.03c (40)
|
0.33 ± 0.03c (25)
|
0.41 ± 0.04c (43)
|
0.42 ± 0.04c (40)
|
|
5
|
C + Riboflavin (16.0 μM)
|
0.64 ± 0.04b (80)
|
0.95 ± 0.03b (72)
|
0.82 ± 0.04b (85)
|
0.76 ± 0.03b (73)
|
|
8-week-old C57BL/6
|
|
1
|
Media + Cells (macrophages) = A
|
0
|
0
|
0
|
0
|
|
2
|
A + LPS (10 ng/well) = B
|
0.35
|
5.32
|
11.67
|
4.97
|
|
3
|
B + 0.4 % DMSO = C
|
0.36 ± 0.02a (100)2
|
6.89 ± 0.86a (100)2
|
12.34 ± 1.1a (100)2
|
5.67 ± 0.67a (100)2
|
|
4
|
C + δ-Tocotrienol (40.0 μM)
|
0.16 ± 0.08c (45)
|
2.34 ± 0.42c (34)
|
1.98 ± 0.24c (16)
|
2.12 ± 0.56c (56)
|
|
5
|
C + Riboflavin (40.0 μM)
|
0.22 ± 0.02b (61)
|
4.15 ± 0.68b (60)
|
7.23 ± 0.98b (59)
|
4.16 ± 0.53b (73)
|
|
8-week-old BALB/c
|
|
1
|
Media + Cells (macrophages) = A
|
0
|
0
|
0
|
0
|
|
2
|
A + LPS (10 ng/well) = B
|
0.62
|
8.27
|
14.45
|
7.76
|
|
3
|
B + 0.4 % DMSO = C
|
0.74 ± 0.05a (100)2
|
10.67 ± 1.24a (100)2
|
15.67 ± 1.45a (100)2
|
9.85 ± 0.88a (100)2
|
|
4
|
C + δ-Tocotrienol (40.0 μM)
|
0.39 ± 0.01c (53)
|
4.16 ± 0.36c (39)
|
3.45 ± 0.28c (22)
|
6.64 ± 0.86b (67)
|
|
5
|
C + Riboflavin (40.0 μM)
|
0.54 ± 0.03b (73)
|
6.45 ± 0.91b (60)
|
4.73 ± 0.22b (30)
|
7.64 ± 0.67b (78)
|
- 1RAW 264.7 cells or thioglycolate-elicited peritoneal macrophages from 8-week-old C57BL/6 or BALB/c mice were treated with δ-tocotrienol or riboflavin (16 μM or 40 μM) for 1 h, followed by treatment with LPS (10 ng/well) for 4 h. Total RNA was extracted, reverse transcribed, and the resulting DNA was amplified and analyzed by real time PCR to quantitate expression of TNF-α, IL-1β, IL-6, and iNOS genes [5, 21].
- Cell viability was >95 % in all the treatments. Data are means ± SD, n = 3 (triplicate analysis of each sample).
- 2Percentages of digital values of relative optical density of each cytokine/β-actin of each treatment are in parenthesis. a-cValues in a column not sharing a common superscript letter are significantly different at P < 0.05.
