Table 6 Inhibitory effects of quercetin, riboflavin, resveratrol, pterostilbene, morin hydrate and nicotinic acid with or without δ-tocotrienol on the secretion of TNF-α and production of nitric oxide by LPS-induced peritoneal macrophages from C57BL/6 mice 1
NO | Assay mixture | Concentration | Serum concentration | Serum concentration |
|---|---|---|---|---|
in μM | of TNF-α (pg/mL) | of nitric oxide (μM) | ||
1 | Medium + Cells = A | 0 | 0 | |
2 | A + LPS (10 ng/well) = B | 1098.87 | 28.89 | |
3 | B + 0.2 % DMSO2 = C | 1056.23 (100)3 | 28.54 (100)3 | |
4 | C + δ-Tocotrienol = D | 5.0 | 674.56 (64) | 18.98 (67) |
5 | C + Quercetin | 40.0 | 589.98 (56) | 20.43 (72) |
6 | C + Riboflavin | 40.0 | 502.87 (58) | 22.54 (79) |
7 | C + Resveratrol | 40.0 | 542.76 (51) | 17.56 (61) |
8 | C + Pterostilbene | 40.0 | 567.34 (54) | 16.87 (59) |
9 | C + Morin hydrate | 40.0 | 597.42 (57) | 19.65 (69) |
10 | C + Nicotinic acid | 40.0 | 727.71 (69) | 23.89 (84) |
11 | B + 0.2 % DMSO = C | 1062.45 (100) | 27.98 (100) | |
12 | D + Quercetin | 40.0 | 432.87 (41) | 14.76 (53) |
13 | D + Riboflavin | 40.0 | 465.21 (44) | 17.43 (62) |
14 | D + Resveratrol | 40.0 | 346.76 (33) | 12.67 (45) |
15 | D + Pterostilbene | 40.0 | 412.45 (39) | 13.25 (47) |
16 | D + Morin hydrate | 40.0 | 432.67 (41) | 15.32 (55) |
17 | D + Nicotinic acid | 40.0 | 588.56 (55) | 18.96 (68) |