Skip to main content

Table 6 Inhibitory effects of quercetin, riboflavin, resveratrol, pterostilbene, morin hydrate and nicotinic acid with or without δ-tocotrienol on the secretion of TNF-α and production of nitric oxide by LPS-induced peritoneal macrophages from C57BL/6 mice 1

From: Inhibition of nitric oxide and inflammatory cytokines in LPS-stimulated murine macrophages by resveratrol, a potent proteasome inhibitor

NO Assay mixture Concentration Serum concentration Serum concentration
in μM of TNF-α (pg/mL) of nitric oxide (μM)
1 Medium + Cells = A   0 0
2 A + LPS (10 ng/well) = B   1098.87 28.89
3 B + 0.2 % DMSO2 = C   1056.23 (100)3 28.54 (100)3
4 C + δ-Tocotrienol = D 5.0 674.56 (64) 18.98 (67)
5 C + Quercetin 40.0 589.98 (56) 20.43 (72)
6 C + Riboflavin 40.0 502.87 (58) 22.54 (79)
7 C + Resveratrol 40.0 542.76 (51) 17.56 (61)
8 C + Pterostilbene 40.0 567.34 (54) 16.87 (59)
9 C + Morin hydrate 40.0 597.42 (57) 19.65 (69)
10 C + Nicotinic acid 40.0 727.71 (69) 23.89 (84)
11 B + 0.2 % DMSO = C   1062.45 (100) 27.98 (100)
12 D + Quercetin 40.0 432.87 (41) 14.76 (53)
13 D + Riboflavin 40.0 465.21 (44) 17.43 (62)
14 D + Resveratrol 40.0 346.76 (33) 12.67 (45)
15 D + Pterostilbene 40.0 412.45 (39) 13.25 (47)
16 D + Morin hydrate 40.0 432.67 (41) 15.32 (55)
17 D + Nicotinic acid 40.0 588.56 (55) 18.96 (68)
  1. 1Thioglycolate-elicited peritoneal macrophages from 8-week-old C57BL/6 mice were pre treated with quercetin, riboflavin, resveratrol, pterostilbene, morin hydrate, or nicotinic acid (40 μM) with or without δ-tocotrienol (5 μM) for 1 h, followed by treatment with LPS (10 ng/well). After 4 h (TNF-α) or 36 h (NO) of LPS challenge, supernatants were collected and assayed for TNF-α and NO, respectively. Cell viability was >95% in all the treatments.
  2. 2DMSO = dimethyl sulfuoxide.
  3. 3Percentages based on actual values of TNF-α (pg/mL) and NO (μM), compared to controls, are in parentheses.