|
NO
|
Assay mixture
|
Concentration
|
Serum concentration
|
Serum concentration
|
|---|
|
in μM
|
of TNF-α (pg/mL)
|
of nitric oxide (μM)
|
|---|
|
1
|
Medium + Cells = A
| |
0
|
0
|
|
2
|
A + LPS (10 ng/well) = B
| |
1098.87
|
28.89
|
|
3
|
B + 0.2 % DMSO2 = C
| |
1056.23 (100)3
|
28.54 (100)3
|
|
4
|
C + δ-Tocotrienol = D
|
5.0
|
674.56 (64)
|
18.98 (67)
|
|
5
|
C + Quercetin
|
40.0
|
589.98 (56)
|
20.43 (72)
|
|
6
|
C + Riboflavin
|
40.0
|
502.87 (58)
|
22.54 (79)
|
|
7
|
C + Resveratrol
|
40.0
|
542.76 (51)
|
17.56 (61)
|
|
8
|
C + Pterostilbene
|
40.0
|
567.34 (54)
|
16.87 (59)
|
|
9
|
C + Morin hydrate
|
40.0
|
597.42 (57)
|
19.65 (69)
|
|
10
|
C + Nicotinic acid
|
40.0
|
727.71 (69)
|
23.89 (84)
|
|
11
|
B + 0.2 % DMSO = C
| |
1062.45 (100)
|
27.98 (100)
|
|
12
|
D + Quercetin
|
40.0
|
432.87 (41)
|
14.76 (53)
|
|
13
|
D + Riboflavin
|
40.0
|
465.21 (44)
|
17.43 (62)
|
|
14
|
D + Resveratrol
|
40.0
|
346.76 (33)
|
12.67 (45)
|
|
15
|
D + Pterostilbene
|
40.0
|
412.45 (39)
|
13.25 (47)
|
|
16
|
D + Morin hydrate
|
40.0
|
432.67 (41)
|
15.32 (55)
|
|
17
|
D + Nicotinic acid
|
40.0
|
588.56 (55)
|
18.96 (68)
|
- 1Thioglycolate-elicited peritoneal macrophages from 8-week-old C57BL/6 mice were pre treated with quercetin, riboflavin, resveratrol, pterostilbene, morin hydrate, or nicotinic acid (40 μM) with or without δ-tocotrienol (5 μM) for 1 h, followed by treatment with LPS (10 ng/well). After 4 h (TNF-α) or 36 h (NO) of LPS challenge, supernatants were collected and assayed for TNF-α and NO, respectively. Cell viability was >95% in all the treatments.
- 2DMSO = dimethyl sulfuoxide.
- 3Percentages based on actual values of TNF-α (pg/mL) and NO (μM), compared to controls, are in parentheses.
