Long-term treatment with the pan-PPAR agonist tetradecylthioacetic acid or fish oil is associated with increased cardiac content of n-3 fatty acids in rat

Table 4 Gene expression of selected genes in heart of rats after 50 weeks of diet administration ^a

		Dietary supplementation^b			Statistical significance of variance ratio (P)^c, effects of
	Control	TTA	FO	TTA + FO	TTA	FO	TTA*FO
Cpt-Ib	1.00 ± 0.17^d	1.29 ± 0.17	1.29 ± 0.19	1.34 ± 0.14	0.003	0.003	0.04
Ucp2	1.00 ± 0.17	0.92 ± 0.29	1.04 ± 0.17	0.74 ± 0.18	0.008	0.29	0.11
Ucp3	1.00 ± 0.43	2.08 ± 0.76	1.74 ± 0.50	2.31 ± 0.62	<0.001	0.01	0.18
Pgc1α	1.00 ± 0.10	0.99 ± 0.16	1.04 ± 0.19	1.05 ± 0.21	0.95	0.36	0.85
Tfam	1.00 ± 0.08	0.99 ± 0.10	0.98 ± 0.11	0.90 ± 0.15	0.23	0.11	0.33
Nrf1	1.00 ± 0.14	1.06 ± 0.11	1.21 ± 0.12	0.98 ± 0.16	0.06	0.13	0.002
Cd36	1.00 ± 0.14	0.94 ± 0.13	1.09 ± 0.24	0.99 ± 0.10	0.14	0.20	0.68
Cact	1.00 ± 0.14	1.26 ± 0.30	1.18 ± 0.17	1.37 ± 0.22	0.002	0.04	0.61
Fabp3	1.00 ± 0.10	1.15 ± 0.37	1.23 ± 0.17	1.24 ± 0.14	0.26	0.03	0.32
Acads	1.00 ± 1.14	2.31 ± 1.69	1.92 ± 1.91	0.83 ± 1.22	0.82	0.56	0.02
Acadm	1.00 ± 0.18	1.15 ± 0.28	1.14 ± 0.28	1.13 ± 0.26	0.43	0.45	0.32
Acadvl	1.00 ± 0.15	1.12 ± 0.20	1.14 ± 0.16	1.05 ± 0.34	0.80	0.64	0.15
Pparα	1.00 ± 0.34	0.88 ± 0.14	1.15 ± 0.33	1.03 ± 0.20	0.16	0.09	0.97
Pparδ	1.00 ± 0.18	0.87 ± 0.09	1.12 ± 0.17	0.90 ± 0.19	0.002	0.15	0.39
Pparγ	1.00 ± 0.28	0.83 ± 0.16	1.33 ± 0.34	0.87 ± 0.17	<0.001	0.02	0.07
Fatp1	1.00 ± 0.28	1.25 ± 0.25	1.55 ± 0.53	1.50 ± 0.31	0.39	0.001	0.19

Abbreviations: TTA, tetradecylthioacetic acid; FO, fish oil; Cpt-Ib, carnitine palmitoyltransferase Ib; Ucp2, uncoupling protein 2; Ucp3, uncoupling protein 3; Pgc1α, peroxisome proliferative activated receptor (gamma coactivator 1); Tfam, transcription factor A mitochondrial; Nrf1, nuclear respiratory factor; Cd36, cluster of differentiation 36; Cact, carnitine-acylcarnitine translocase; Fabp3, fatty acid binding protein 3; Acads, acyl-CoA dehydrogenase short chain; Acadm, acyl-CoA dehydrogenase medium chain; Acadvl, acyl-CoA dehydrogenase very long chain; Pparα, peroxisome proliferator-activated receptor α; Pparδ, peroxisome proliferator-activated receptor d; Pparγ, peroxisome proliferator-activated receptor γ; Fatp1, fatty acid transport protein 1.
^a Relative to control diet.
^b n=10 in each group.
^c P-values from two-way ANOVA.
^d Values are mean ± SD.

ISSN: 1476-511X