Figure 5

Influence of method of HDL isolation on HDL-DCFH interactions. DCFH was exposed to 2.5 μg (cholesterol) of FPLC-purified (FPLC) or ultracentrifugation-purified HDL (UC) that was isolated from 10 patients with coronary artery disease (CAD) and 10 healthy volunteers (Non CAD) as described in Materials and Methods. Each sample was run in quadruplicate. The median slopes of oxidation are expressed as % oxidation slopes compared to the median slope of oxidation of DCFH. The median oxidation rate of DCFH was significantly higher when HDL isolated by ultracentrifugation was added compared to when HDL isolated by FPLC was added (p < 0.05, n = 20). * p < 0.05.