Figure 7

HDL was isolated from plasma (with heparin or citrate as anticoagulants) and serum of 12 different healthy volunteers, using ultracentrifugation. Oxidation of DHR in the presence of these 12 different HDL samples was assessed as described in Methods, using 2.5 μg (cholesterol) of added HDL. The slope of oxidation of each sample was normalized to the slope of oxidation of DHR and the % relative oxidation slope and quenching is shown on the y axis. The data (means of quadruplicates) from three independent experiments are plotted. We found that the HDL samples isolated from plasma with heparin and from sera had significantly (p < 0.05) higher DOR values (78.7% ±6.8 and 79.8 ± 4.9 respectively) compared to the same HDL samples isolated from plasma citrate (66.2% ±6.1).