Figure 4

Representative erythrocyte images under scanning electron microscopy. In the Figure 4 A1 and B1, two figures demonstrated that the lowest dose of flaxseed oil exerted minor ability on membrane. Many bulges gradually emerged on membrane (red arrows, 20000× and 25000×). In the Figure 4 A2 and B2, 100 μM flaxseed oil group was better than 50 μM flaxseed oil group, the membrane gradually became to smooth and bulges also gradually alleviated on membrane (red arrows, 20000× and 25000×). In the Figure 4 A3 and B3 (20000× and 25000×), the 200 μM flaxseed oil group was similar as PBS group (Figure 4 B4, 20000×), the normal shape and normal membrane in PBS group. In the Figure 4 A4, the erythrocytes in positive group (50 mM glucose without flaxseed oil). The blood cells became flat cells (leptocyte) and acanthoid cells (25000×). They basically lost their normal shape.