Figure 6From: Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient miceLocalization of endothelial progenitor cells (EPC) and green fluorescent protein (GFP)-derived mononuclear cells (MNC). The photomicrographs are typical common carotid cross-sections stained for the markers Sca-1-PE (stem cell antigen 1-phycoerythrin) and CD117-FITC (stem cell growth factor receptor-fluorescein isothiocyanate), showing an intense immunofluorescent reaction in MNC-treated apoE-/- animals (indicated by the arrows and merged image) in contrast with the lack of fluorescence in the Cuff-Vehicle mice. The self-fluorescence of the MNC-derived GFP confirms the presence of the MNCs in the endothelial vessel of the treated (but not the untreated) mouse and indicates that the treatment was able to recruit endogenous EPCs. Scale bar: 100 μm.Back to article page