Figure 1From: Omega-3 and omega-6 PUFAs induce the same GPR120-mediated signalling events, but with different kinetics and intensity in Caco-2 cellsDetection of GPR120 and GPR40 mRNA using PCR. Total mRNA was isolated from tissue samples and Caco-2 cells using Qiagen RNase Mini Kit. Rplp0 is a housekeeping gene, and was used as a control. Each sample was analyzed in duplicates. The PCR products were visualised using 1% agarose gel electrophoresis.Back to article page