Effect of PUFAs on [Ca2+]
in Caco-2 cells. (A) Traces represent the effect of treating Caco-2 cells with 200 μM EPA, DHA and AA. F360/F380 is the ratio between emissions at the two different excitation wavelengths and reflects [Ca2+]i. PUFAs were applied for 10–30 sec from the time indicated by the vertical line. (B) The maximum [Ca2+]i response obtained after treatment with the different PUFAs, estimated as % increase above baseline. (C) The time to peak of [Ca2+]i, measured as the time between 10% and 90% of maximum increase of [Ca2+]i. (D) The delay in seconds from start of treatment with indicated PUFAs until the increase of [Ca2+]i had reached 10% of maximum [Ca2+]i. Data is presented as mean ± SEM from n = 15 (EPA), 14 (DHA) and 10 (AA) cells.