Figure 4

The effect of PUFAs on IL-1β induced degradation of IκBα in Caco-2 cells. (A) Cells were treated with 100 μM EPA, DHA, AA for 1 h, or with 100 ng/ml IL-1β for 30 min, or with 100 μM EPA, DHA, AA for 1 hour and then treated with 100 ng/ml IL-1β for 30 min. Differences in IκBα expression were determined using densitometry and compared to untreated cells (n = 3, * p < 0.05). (B) Cells were pretreated with 10 μM GW5074 and 100 nM Iressa for 1 h before treatment with 100 μM EPA for 1 h and then treated with 100 ng/ml IL-1β for 30 min. Cells were also treated with only 100 μM EPA, 10 μM GW5074 and 100 nM Iressa for 1 h and 100 ng/ml IL-1β for 30 min. The lysates were subjected to Western blotting to detect total IκBα and actin. The figure is representative for at least three experiments with similar results.