Palmitate induced C2C12 myotube loss. Myotubes were treated with palmitate or/and oleate for 24 hours. (A) Photographs were taken before (light) or after (crystal violet staining) 1% crystal violet staining under a phase contrast microscope; (B) The number of myotubes per photograph was counted after crystal violet staining (n=9). (C) The transcription of myogenin, MHC1, MHC2b and MCK genes was measured by qRT-PCR. The values were monitored by 18S and expressed as mean±SEM (n=3). (D) Photographs were taken like (A), and the number of myotubes per photograph was counted. *p<0.05, **p<0.01 vs. CTL. PA, palmitate; OL, oleate; CTL, control.