Palmitate altered the expression of myokine genes in C2C12 myotbes. Cells were treated with palmitate for 24 hours. The transcription of FNDC5 gene (A), CTRP15 gene (B), FGF21 gene (C) and IL6 gene (D) was measured by qRT-PCR and monitored via 18S. FNDC5 protein level was measured by western blot, GAPDH as control (E). The values were expressed as mean±SEM (n=3). *p<0.05, **p<0.01 vs. CTL.