Effects of PUFAs on intracellular lipid accumulation. Both THP-1 macrophages and monocyte macrophage were pre-treated with different NEFA at the concentration of 100 μM for 72 hours. Then ox-LDL was added to facilitate foam cell formation. The intracellular lipid droplets were stained with Nile Red (A). The average positive area of stained LDs per cell was used to quantify the intracellular lipid content (B). Data represent mean ± SEM (n = 3). ※P < 0.05 vs. control; #P < 0.05 vs. PA/OA group.