Quantitative determination of TG, TC and CE in foam cells. The THP-1 macrophages or monocyte macrophages were pre-treated with different NEFA in advance, and then ox-LDL was added to facilitate foam cell formation. Intracellular TG, TC and free cholesterol were determined by enzymatic colorimetric assays (A-B). The concentration of CE was determined by subtracting free cholesterol from TC (C). Data represent mean ± SEM (n = 3).※/#P < 0.05 vs. control (A); †/‡P < 0.05 vs. PA/OA group (B-C).