Effects of PUFAs on the mRNA expressions of CIDE and PAT members during NEFA-induced LD formation. The THP-1 macrophages were pre-treated with different NEFA in advance, and then ox-LDL was added to facilitate foam cells formation. Total RNAs were extracted from both macrophages and foam cells and reverse-transcribed into cDNA. CIDE (A) and PAT (B) family members were measured by real-time PCR. Data represent mean ± SEM (n = 3). ※P < 0.05 vs. the value in macrophages; †P < 0.05 vs. the un-treated control.