APOE and APOA1 levels in HDL subfractions in control and Pcsk9 KO mice. NHDS obtained from 5 males per genotype was electrophoresed on non-denaturing gels and blotted using antibodies against APOE and APOA1 (A). Fifteen μg of proteins were separated by SDS-PAGE and blotted using antibody against either APOE or APOA1. The relative intensity of the APOE (B) and APOA1 (C) bands is shown. Data represent the mean ± SEM. * P < 0.05, ‡P < 0.0001.