Figure 3From: Decreased APOE-containing HDL subfractions and cholesterol efflux capacity of serum in mice lacking Pcsk9HDL cholesterol concentrations in serum, APOE and APOA1 levels in HDL subfractions in control and Tg(hLDLR) mice. Serum and NHDS were obtained from serum of 8-week-old males (n = 5 per genotype). HDL cholesterol concentration was measured in control and Tg(hLDLR) mice (A). HDL subfractions were electrophoresed in a non-denaturing gel and blotted using antibodies for APOE and APOA1 (B). Fifteen μg of proteins of NHDS were separated by SDS-PAGE and blotted using an APOE antibody. The relative band intensity of APOE is shown (C). Data represent the mean ± SEM. †P < 0.001.Back to article page