Maximum conjugated dienes production in serum from Maués inhabitants. Serum (diluted 100-fold) in PBS 10 mM (pH7.4) was incubated at 37°C for 180 min in the presence of 30 μM CuSO4. Conjugated diene formation was measured by determining the absorbance at 245 nm every 20 min. In panel A, maximum CD production in serum of GI and NG subjects. In panel B, gender effect on maximum CD production between GI and NG groups. NG (white bars) and GI (gray bars). In panel C, the t(1/2) indicates the time required to achieve 50% CD production. * p < 0.05 compared to white bar.