Figure 4
From: Critical role of leukotriene B4 receptor signaling in mouse 3T3-L1 preadipocyte differentiation
Effect of BLT1 and BLT2 knockdown by siRNA on mouse 3T3-L1 preadipocyte differentiation. (A): Confirmation of BLT1 and BLT2 knockdown by specific siRNAs. Negative control is cells treated with negative control siRNA (Stealth RNAi Negative Control Duplexes). (B) and (C): Effect of BLT1 knockdown by siRNA. Representative microscopic (B) images of differentiated mouse 3T3-L1 adipocytes by Oil Red O staining. Scale bar represents 100 μm. (C): Accumulation of TG in mature adipocytes was measured and expressed as TG contents (μg/mg protein). Each column represents the mean ± SEM from 3-5 independent experiments. *P<0.05 vs. negative control-siRNA treatment. (D) and (E): Effect of BLT2 knockdown by siRNA. Representative microscopic (D) images of differentiated mouse 3T3-L1 adipocytes by Oil Red O staining. Scale bar represents 100 μm. (E): Accumulation of TG in mature adipocytes was measured and expressed as TG contents (μg/mg protein).