Figure 5

Combination knockdown of BLT1 and BLT2 by siRNA on mouse 3T3-L1 preadipocyte differentiation. Mouse 3T3-L1 preadipocytes were treated with a combination of BLT1-siRNA (12.5 nM) and BLT2-siRNA (12.5 nM). (A): Representative microscopic images of differentiated mouse 3T3-L1 adipocytes by Oil Red O staining. Scale bar represents 100 μm. (B): Accumulation of TG in mature adipocytes was measured and expressed as TG contents (% of negative control). Each column represents the mean ± SEM from 3 independent experiments. *P<0.05, **P<0.01 vs. negative control-siRNA treatment.