Effects of Cirsium brevicaule A. GRAY leaf (CL) extracts on lipid accumulation in 3T3-L1 adipocytes. (A) Oil red O staining in 3T3-L1 cells treated with each CL extract. The data from one representative experiment out of three are shown. (B) Intracellular triglyceride (TG) content of the 3T3-L1 cells. Each value represents as the means ± SEM for three independent experiments. ND, non-differentiated cells. The asterisks shows significant differences between the experimental groups, determined using Student’s t-test (*: P < 0.05, **: P < 0.01).