Skip to main content
Figure 2 | Lipids in Health and Disease

Figure 2

From: Phenotypic alterations in human saphenous vein culture induced by tumor necrosis factor-alpha and lipoproteins: a preliminary development of an initial atherosclerotic plaque model

Figure 2

Proliferation of SMC in HSV cultured with T + LDL. (A) Nuclear expression of proliferating cell nuclear antigen (PCNA) by immunohistochemical staining; (a), markedly localization of PCNA in nuclei of lymphoblasts in marginal zone of human tonsillitis section (PCNA positive control staining, original magnification x 200); b and c, double immunostaining of HSV rings (original magnification x 400); on day 0 (b) and day 14 after cultured with T (5 ng/ml) + oxLDL (50 μg/ml) (c), PCNA in nuclei and alpha actin in cytoplasm of SMC, inlet with x1000 magnification. PCNA and alpha actin positive reactivity was visualized with peroxidase substrate DAB (brown color) and Nova Red (red color), respectively. (B) Percentages of PCNA expressed SMC in HSV rings were compared among all groups as mentioned above. Data are means ± SEM from 3 - 6 independent experiments. Significant differences among groups are indicated; * p- valves = 0.00, ** p- valves < 0.05, and *** = not significant.

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X