Figure 3

Histological examination of the liver with hematoxylin and eosin (H & E) staining and determining the excessive deposition of glycogen by periodic acid-Schiff (PAS) in rats that started the experiment at four weeks of age (Young; panels A to F) and at 12 weeks (Adult, panels G to L) submitted to AIN-93 (CT), high-fructose (FRU) or high-fat (FAT) diet for 13 weeks. The arrowhead () represents the non-alcoholic microvesicular fatty liver disease (NAFLD), and the long arrow () represents the occurrence of macrovesicular NAFLD. Panels E and F show higher glycogen stores in the Young – FRU and Adult – FAT groups. 40× magnification.