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Figure 1 | Lipids in Health and Disease

Figure 1

From: Modulating the inflammatory properties of activated microglia with Docosahexaenoic acid and Aspirin

Figure 1

A. 50,000 EOC20 Microglial Cells were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with either Aspirin at 1mM or Aspirin at 5mM. At 24 hours post TLR stimulation and/or Aspirin treatment, the experimental plate was assayed for necrosis using a Non-Radioactive Cell Proliferation kit (MTT Assay). Untrtd = Untreated; Asp1 = Aspirin (1mM); Asp5 = Aspirin (5mM); DHA = Docosahexaenoic Acid (50μM). * = p<0.05; ** = p<0.005. B. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with Poly I:C, Aspirin at 1mM, Aspirin at 5mM, or a combination of Poly I:C and Aspirin. At 24 hours post Poly I:C stimulation and/or Aspirin treatment, the experimental plate was assayed for necrosis using a Non-Radioactive Cell Proliferation kit (MTT Assay). Untrtd = Untreated; PIC = Poly I:C (1μg/mL); Asp1 = Aspirin (1mM); Asp5 = Aspirin (5mM); DHA = Docosahexaenoic Acid (50μM). * = p<0.05; ** = p<0.005; *** = p<0.0005. C. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with Imiquimod, Aspirin at 1mM, Aspirin at 5mM, or a combination of Imiquimod and Aspirin. At 24 hours post Imiquimod stimulation and/or Aspirin treatment, the experimental plate was assayed for necrosis using a Non-Radioactive Cell Proliferation kit (MTT Assay). Untrtd = Untreated; Imi = Imiquimod (10μg/mL); Asp1 = Aspirin (1mM); Asp5 = Aspirin (5mM); DHA = Docosahexaenoic Acid (50μM). * = p<0.05; *** = p<0.0005; **** = p<0.00005; ***** = p<0.000005.

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