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Figure 5 | Lipids in Health and Disease

Figure 5

From: Modulating the inflammatory properties of activated microglia with Docosahexaenoic acid and Aspirin

Figure 5

A. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with Poly I:C, 1mM Aspirin, or a combination of Poly I:C and Aspirin. At 24 hours post Poly I:C and/or Aspirin treatment, supernatants were collected; total incubation time was 48 hours. IL-6 ELISAs determined IL-6 secretion. Values were normalized to the untreated. Untrtd = Untreated; PIC = Poly I:C (1μg/mL); Asp = Aspirin (1mM); DHA = Docosahexaenoic Acid (50μM). # = p< 0.05; **,§§ = p<0.005. 5B. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with Imiquimod, 1mM Aspirin, or a combination of Imiquimod and Aspirin. At 24 hours post Imiquimod and/or Aspirin treatment, supernatants were collected; total incubation time was 48 hours. IL-6 ELISAs determined IL-6 secretion. Values were normalized to the untreated. Untrtd = Untreated; Imi = Imiquimod (10μg/mL); Asp = Aspirin (1mM); DHA = Docosahexaenoic Acid (50μM). §§§ = p<0.0005; #### = p<0.00005; ****** = p<0.0000005. 5C. 50,000 EOC20 Microglia were plated and stimulated as in 5A. TNF- α ELISAs determined TNF- α secretion. Values were normalized to the untreated. Untrtd = Untreated; PIC = Poly I:C (1μg/mL); Asp = Aspirin (1mM); DHA = Docosahexaenoic Acid (50μM). ## = p< 0.005; *** = p< 0.0005; §§§§§ = p<0.000005. 5D. 50,000 EOC20 Microglia were plated and stimulated as in 5B. TNF- α ELISAs determined TNF- α secretion. Values were normalized to the untreated. Untrtd = Untreated; Imi = Imiquimod (10μg/mL); Asp = Aspirin (1mM)DHA = Docosahexaenoic Acid (50μM). # = p<0.05; **, §§ = p<0.005.

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