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Figure 7 | Lipids in Health and Disease

Figure 7

From: Modulating the inflammatory properties of activated microglia with Docosahexaenoic acid and Aspirin

Figure 7

A. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μΜ aspirin or a combination of aspirin and Poly I:C. At 24 hours post Poly I:C and/or asprin treatment, supernatants were collected; total incubation time was 48 hrs. Supernatants were assayed for total Nitric Oxide using a total Nitric Oxide kit. Values were normalized to the untreated. Untrtrd=Untreated; PIC=Poly I:C (1μg/mL); Asp = Aspirin (1mM); DHA = Docosahexaenoic Acid (50μM). **, ## = p<0.005; *** = p<0.0005; §§§§§ = p<0.00005. B. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with Imiquimod, 1mM Aspirin, or a combination of Imiquimod and Aspirin. At 24 hours post Imiquimod and/or Aspirin treatment, supernatants were collected; total incubation time was 48 hours. Supernatants were assayed for total Nitric Oxide content using a total Nitric Oxide kit. Values were normalized to the untreated. Untrtd = Untreated; Imi = Imiquimod (10μg/mL); Asp = Aspirin (1mM); DHA = Docosahexaenoic Acid (50μM). ** = p<0.005; §§§ = p<0.0005.

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