Table 3 Fatty acid content and fold increase in LA, GLA, AA, ALA, EPA and DHA in RWPE-1 and PC-3 cells supplemented with these fatty acids as compared to control

Control - LA

0.38 ± 0.09

-

0.14 ± 0.01

-

LA 50 μM

1.59 ± 0.13

4.18

0.80 ± 0.10

5.71

LA 150 μM

0.52 ± 0.18

1.37

1.92 ± 0.38

13.71

Control - GLA

0.76 ± 0.13

-

0.88 ± 0.03

-

GLA 25 μM

1.44 ± 0.09

1.89

0.97 ± 0.08

1.10

GLA 50 μM

1.01 ± 0.13

1.33

1.08 ± 0.28

1.23

GLA 75 μM

12.34 ± 0.55

17.63

0.76 ± 0.01

< 1.0

Control- AA

0.92 ± 0.14

-

1.22 ± 0.03

-

AA 50 μM

7.01 ± 0.74

7.62

14.28 ± 0.44

11.70

AA 100 μM

10.28 ± 0.43

11.17

22.20 ± 0.63

18.20

Control- ALA

0.47 ± 0.09

-

0.12 ±0.01

-

ALA 50 μM

0.62 ± 0.13

1.32

0.51 ± 0.13

4.25

ALA 150 μM

0.86 ± 0.10

1.83

2.13 ± 0.18

17.75

Control- EPA

0.86 ± 0.13

-

N.D.

-

EPA 50 μM

1.14 ± 0.25

1.33

2.78 ± 0.03

>2.78

EPA 100 μM

4.42 ± 0.43

3.88

5.64 ± 1.80

>5.64

Control-DHA

2.58 ± 0.49

-

0.71 ± 0.03

-

DHA 50 μM

14.95 ± 0.63

5.79

11.17 ± 1.08

15.73

DHA 100 μM

15.35 ± 0.08

5.95

19.08 ±0.06

26.87