Effects of pomegranate vinegar or acetic acid supplementation on the p-AMPK protein (A), PPARα (B), CPT-1a (C), SREBP-1c (D), ACO (E), UCP2 (F) mRNA levels in the liver of SD rats fed a HF diet for 16 weeks ( n = 10 per group). Representative western blots of adipose tissue lysate with antibodies against AMPK, p-AMPK and β-actin as loading control. Total protein (40 ug) from the liver was electrophoresed on to SDS-phage gel and transferred to PVDF membranes. The effects of dietary PV or acetic acid supplementation on hepatic key lipolysis enzyme mRNA levels were examined by real-time PCR. Data are shown as the mean ± SEM. abcMeans not sharing common letters are significantly different between groups at p < 0.05.