Skip to main content
Figure 2 | Lipids in Health and Disease

Figure 2

From: Reduced beta2-glycoprotein I protects macrophages from ox-LDL-induced foam cell formation and cell apoptosis

Figure 2

Intracellular cholesterol content of RAW264.7 macrophage. (A) The content of intracellular TC, FC and CE. (B) The ratio of CE to TC. RAW264.7 macrophages were seeded onto 96-well microtitre plates and then supplemented with ox-LDL alone or along with beta2-GPI or reduced beta2-GPI. HPLC-MS method was used to quantitatively determine the content of free cholesterol. Total protein was detected by the BCA method, and the final cholesterol content was determined using the ratio between the cholesterol concentration and the corresponding protein concentration (μg/mg protein). Ox-LDL increased the content of TC, FC, CE and the value of CE/TC. Beta2-GPI or reduced beta2-GPI decreased TC content in macrophages, whereas latter caused a larger decrease than the former. Note: n = 3, TC, total cholesterol; FC, free cholesterol; CE, cholesterol ester. *: compared with the control group, p < 0.01; ▲: compared with the ox-LDL group, p < 0.05; ▲▲: compared with the ox-LDL group, p < 0.01; #: compared with the beta2-GPI group, p < 0.05.

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X