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Table 1 Weight % of fatty acids in dried blood spots at varying BHT concentrations over 28 days

From: Butylated hydroxytoluene can protect polyunsaturated fatty acids in dried blood spots from degradation for up to 8 weeks at room temperature

Name 0 d 1 d 3 d 7 d 14 d 21 d 28 d
  0 mg/mL BHT
SFAs 42.1 ± 0.9a 40.2 ± 0.5a 49.2 ± 0.6b 54.1 ± 1.7c 56.0 ± 0.3c 54.3 ± 0.5c 54.9 ± 0.9c
MUFAs 18.0 ± 0.4a 18.7 ± 0.2a 22.3 ± 0.3c 21.3 ± 0.8bc 20.8 ± 0.3b 21.8 ± 0.4bc 22.3 ± 0.7c
N-6 FAs 34.0 ± 0.6a 35.4 ± 0.1b 23.9 ± 0.3c 20.1 ± 0.5d 18.3 ± 0.1e 19.0 ± 0.3de 18.2 ± 0.6e
N-3 FAs 4.17 ± 0.24a 4.14 ± 0.03a 1.98 ± 0.11b 1.45 ± 0.08c 1.30 ± 0.13c 1.63 ± 0.20bc 1.47 ± 0.15c
ARA 8.07 ± 0.14a 8.10 ± 0.17a 4.25 ± 0.07b 3.20 ± 0.14c 2.85 ± 0.07c 3.17 ± 0.10c 2.92 ± 0.22c
EPA 0.65 ± 0.10a 0.64 ± 0.04a 0.29 ± 0.02b 0.26 ± 0.03b 0.25 ± 0.05b 0.25 ± 0.02b 0.17 ± 0.02b
DHA 2.19 ± 0.09a 2.18 ± 0.03a 0.85 ± 0.05b 0.63 ± 0.04cd 0.55 ± 0.02d 0.73 ± 0.02cd 0.77 ± 0.06cd
PUFAs 38.1 ± 0.8a 39.5 ± 0.1b 25.9 ± 0.3c 21.5 ± 0.5d 19.6 ± 0.1e 20.6 ± 0.5de 19.6 ± 0.4e
HUFAs 14.1 ± 0.5a 14.1 ± 0.2a 7.59 ± 0.10b 5.60 ± 0.24cd 5.02 ± 0.22d 5.80 ± 0.08c 5.40 ± 0.19cd
  2.5 mg/mL BHT
SFAs 37.1 ± 0.5a 37.1 ± 0.3a 37.6 ± 0.5ab 36.2 ± 0.7a 39.2 ± 0.2c 39.0 ± 0.8bc 40.9 ± 0.7d
MUFAs 19.0 ± 0.2ab 18.7 ± 0.4a 19.1 ± 0.4ab 19.8 ± 0.4c 19.6 ± 0.1bc 20.2 ± 0.2cd 20.7 ± 0.1d
N-6 FAs 36.7 ± 0.3a 36.0 ± 0.3a 35.9 ± 0.1a 36.3 ± 0.4a 34.9 ± 0.5b 34.8 ± 0.4b 32.3 ± 0.3c
N-3 FAs 4.43 ± 0.10a 4.32 ± 0.11ab 4.15 ± 0.17abc 4.35 ± 0.12ab 3.96 ± 0.07bc 3.79 ± 0.25c 2.84 ± 0.06d
ARA 9.12 ± 0.07a 8.43 ± 0.24b 8.28 ± 0.08b 8.40 ± 0.12b 8.08 ± 0.07b 7.56 ± 0.22c 5.98 ± 0.13d
EPA 0.71 ± 0.08a 0.73 ± 0.06a 0.68 ± 0.06a 0.66 ± 0.03ab 0.62 ± 0.02ab 0.63 ± 0.07ab 0.52 ± 0.01b
DHA 2.40 ± 0.19a 2.25 ± 0.10ab 2.17 ± 0.06ab 2.35 ± 0.06a 2.16 ± 0.05ab 1.95 ± 0.20b 1.42 ± 0.05c
PUFAs 41.1 ± 0.2a 40.3 ± 0.3ab 40.0 ± 0.3b 40.6 ± 0.4ab 38.9 ± 0.5c 38.6 ± 0.6c 35.1 ± 0.2d
HUFAs 15.7 ± 0.1a 14.8 ± 0.2bc 14.4 ± 0.2bc 14.9 ± 0.2b 14.2 ± 0.1c 13.4 ± 0.5d 10.4 ± 0.2e
  5 mg/mL BHT
SFAs 36.6 ± 0.2 36.9 ± 0.4 37.6 ± 0.8 36.0 ± 0.4 37.9 ± 2.6 38.5 ± 0.9 39.0 ± 0.7
MUFAs 18.6 ± 0.2a 19.0 ± 0.1a 19.2 ± 0.2ab 19.5 ± 0.3ab 21.6 ± 2.4b 20.1 ± 0.6ab 20.4 ± 0.1ab
N-6 FAs 36.0 ± 0.5ab 36.7 ± 0.1a 35.7 ± 0.4abc 36.0 ± 0.5ab 34.2 ± 0.3c 34.8 ± 1.1bc 34.2 ± 0.2c
N-3 FAs 4.59 ± 0.28a 4.23 ± 0.07ab 4.07 ± 0.02bc 4.32 ± 0.08ab 3.73 ± 0.26c 4.16 ± 0.15abc 3.75 ± 0.13c
ARA 8.68 ± 0.15a 8.54 ± 0.19a 8.21 ± 0.11ab 8.53 ± 0.20a 7.60 ± 0.45b 8.35 ± 0.12a 7.72 ± 0.09ab
EPA 0.82 ± 0.08a 0.71 ± 0.03ab 0.62 ± 0.01b 0.66 ± 0.04b 0.60 ± 0.01b 0.62 ± 0.02b 0.61 ± 0.04b
DHA 2.41 ± 0.15a 2.28 ± 0.02a 2.16 ± 0.03ab 2.35 ± 0.03a 1.98 ± 0.19b 2.25 ± 0.11ab 1.96 ± 0.06b
PUFAs 40.6 ± 0.3a 41.0 ± 0.2a 39.7 ± 0.4ab 40.4 ± 0.6ab 37.9 ± 0.6c 39.0 ± 1.0bc 38.0 ± 0.2c
HUFAs 15.5 ± 0.2a 14.8 ± 0.3ab 14.3 ± 0.2bc 15.0 ± 0.3ab 13.2 ± 0.7d 14.8 ± 0.3ab 13.5 ± 0.1cd
  1. Values are means ± SD, n = 3. BHT, butylated hydroxytoluene; SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; FA, fatty acid; n-6, omega-6; n-3, omega-3; ARA, arachidonic acid; EPA, eicosapentaenoic acid; DHA, docosahexaenoic acid; PUFA, polyunsaturated fatty acid; HUFA, highly unsaturated fatty acid. Values with different superscript letters within a row are significantly different by Tukey’s HSD post hoc procedure (P < 0.05) after a significant F-value by one-way ANOVA (P < 0.05).