Butylated hydroxytoluene can protect polyunsaturated fatty acids in dried blood spots from degradation for up to 8 weeks at room temperature

Metherel, Adam H; Hogg, Ryan C; Buzikievich, Lindy M; Stark, Ken D

doi:10.1186/1476-511X-12-22

Lipids in Health and Disease

Table 1 Weight % of fatty acids in dried blood spots at varying BHT concentrations over 28 days

From: Butylated hydroxytoluene can protect polyunsaturated fatty acids in dried blood spots from degradation for up to 8 weeks at room temperature

Name	0 d	1 d	3 d	7 d	14 d	21 d	28 d
	*0 mg/mL BHT*
SFAs	42.1 ± 0.9^a	40.2 ± 0.5^a	49.2 ± 0.6^b	54.1 ± 1.7^c	56.0 ± 0.3^c	54.3 ± 0.5^c	54.9 ± 0.9^c
MUFAs	18.0 ± 0.4^a	18.7 ± 0.2^a	22.3 ± 0.3^c	21.3 ± 0.8^bc	20.8 ± 0.3^b	21.8 ± 0.4^bc	22.3 ± 0.7^c
N-6 FAs	34.0 ± 0.6^a	35.4 ± 0.1^b	23.9 ± 0.3^c	20.1 ± 0.5^d	18.3 ± 0.1^e	19.0 ± 0.3^de	18.2 ± 0.6^e
N-3 FAs	4.17 ± 0.24^a	4.14 ± 0.03^a	1.98 ± 0.11^b	1.45 ± 0.08^c	1.30 ± 0.13^c	1.63 ± 0.20^bc	1.47 ± 0.15^c
ARA	8.07 ± 0.14^a	8.10 ± 0.17^a	4.25 ± 0.07^b	3.20 ± 0.14^c	2.85 ± 0.07^c	3.17 ± 0.10^c	2.92 ± 0.22^c
EPA	0.65 ± 0.10^a	0.64 ± 0.04^a	0.29 ± 0.02^b	0.26 ± 0.03^b	0.25 ± 0.05^b	0.25 ± 0.02^b	0.17 ± 0.02^b
DHA	2.19 ± 0.09^a	2.18 ± 0.03^a	0.85 ± 0.05^b	0.63 ± 0.04^cd	0.55 ± 0.02^d	0.73 ± 0.02^cd	0.77 ± 0.06^cd
PUFAs	38.1 ± 0.8^a	39.5 ± 0.1^b	25.9 ± 0.3^c	21.5 ± 0.5^d	19.6 ± 0.1^e	20.6 ± 0.5^de	19.6 ± 0.4^e
HUFAs	14.1 ± 0.5^a	14.1 ± 0.2^a	7.59 ± 0.10^b	5.60 ± 0.24^cd	5.02 ± 0.22^d	5.80 ± 0.08^c	5.40 ± 0.19^cd
	*2.5 mg/mL BHT*
SFAs	37.1 ± 0.5^a	37.1 ± 0.3^a	37.6 ± 0.5^ab	36.2 ± 0.7^a	39.2 ± 0.2^c	39.0 ± 0.8^bc	40.9 ± 0.7^d
MUFAs	19.0 ± 0.2^ab	18.7 ± 0.4^a	19.1 ± 0.4^ab	19.8 ± 0.4^c	19.6 ± 0.1^bc	20.2 ± 0.2^cd	20.7 ± 0.1^d
N-6 FAs	36.7 ± 0.3^a	36.0 ± 0.3^a	35.9 ± 0.1^a	36.3 ± 0.4^a	34.9 ± 0.5^b	34.8 ± 0.4^b	32.3 ± 0.3^c
N-3 FAs	4.43 ± 0.10^a	4.32 ± 0.11^ab	4.15 ± 0.17^abc	4.35 ± 0.12^ab	3.96 ± 0.07^bc	3.79 ± 0.25^c	2.84 ± 0.06^d
ARA	9.12 ± 0.07^a	8.43 ± 0.24^b	8.28 ± 0.08^b	8.40 ± 0.12^b	8.08 ± 0.07^b	7.56 ± 0.22^c	5.98 ± 0.13^d
EPA	0.71 ± 0.08^a	0.73 ± 0.06^a	0.68 ± 0.06^a	0.66 ± 0.03^ab	0.62 ± 0.02^ab	0.63 ± 0.07^ab	0.52 ± 0.01^b
DHA	2.40 ± 0.19^a	2.25 ± 0.10^ab	2.17 ± 0.06^ab	2.35 ± 0.06^a	2.16 ± 0.05^ab	1.95 ± 0.20^b	1.42 ± 0.05^c
PUFAs	41.1 ± 0.2^a	40.3 ± 0.3^ab	40.0 ± 0.3^b	40.6 ± 0.4^ab	38.9 ± 0.5^c	38.6 ± 0.6^c	35.1 ± 0.2^d
HUFAs	15.7 ± 0.1^a	14.8 ± 0.2^bc	14.4 ± 0.2^bc	14.9 ± 0.2^b	14.2 ± 0.1^c	13.4 ± 0.5^d	10.4 ± 0.2^e
	*5 mg/mL BHT*
SFAs	36.6 ± 0.2	36.9 ± 0.4	37.6 ± 0.8	36.0 ± 0.4	37.9 ± 2.6	38.5 ± 0.9	39.0 ± 0.7
MUFAs	18.6 ± 0.2^a	19.0 ± 0.1^a	19.2 ± 0.2^ab	19.5 ± 0.3^ab	21.6 ± 2.4^b	20.1 ± 0.6^ab	20.4 ± 0.1^ab
N-6 FAs	36.0 ± 0.5^ab	36.7 ± 0.1^a	35.7 ± 0.4^abc	36.0 ± 0.5^ab	34.2 ± 0.3^c	34.8 ± 1.1^bc	34.2 ± 0.2^c
N-3 FAs	4.59 ± 0.28^a	4.23 ± 0.07^ab	4.07 ± 0.02^bc	4.32 ± 0.08^ab	3.73 ± 0.26^c	4.16 ± 0.15^abc	3.75 ± 0.13^c
ARA	8.68 ± 0.15^a	8.54 ± 0.19^a	8.21 ± 0.11^ab	8.53 ± 0.20^a	7.60 ± 0.45^b	8.35 ± 0.12^a	7.72 ± 0.09^ab
EPA	0.82 ± 0.08^a	0.71 ± 0.03^ab	0.62 ± 0.01^b	0.66 ± 0.04^b	0.60 ± 0.01^b	0.62 ± 0.02^b	0.61 ± 0.04^b
DHA	2.41 ± 0.15^a	2.28 ± 0.02^a	2.16 ± 0.03^ab	2.35 ± 0.03^a	1.98 ± 0.19^b	2.25 ± 0.11^ab	1.96 ± 0.06^b
PUFAs	40.6 ± 0.3^a	41.0 ± 0.2^a	39.7 ± 0.4^ab	40.4 ± 0.6^ab	37.9 ± 0.6^c	39.0 ± 1.0^bc	38.0 ± 0.2^c
HUFAs	15.5 ± 0.2^a	14.8 ± 0.3^ab	14.3 ± 0.2^bc	15.0 ± 0.3^ab	13.2 ± 0.7^d	14.8 ± 0.3^ab	13.5 ± 0.1^cd

Values are means ± SD, n = 3. BHT, butylated hydroxytoluene; SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; FA, fatty acid; n-6, omega-6; n-3, omega-3; ARA, arachidonic acid; EPA, eicosapentaenoic acid; DHA, docosahexaenoic acid; PUFA, polyunsaturated fatty acid; HUFA, highly unsaturated fatty acid. Values with different superscript letters within a row are significantly different by Tukey’s HSD post hoc procedure (P < 0.05) after a significant F-value by one-way ANOVA (P < 0.05).

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