Figure 1
From: MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
MiR-613 decreases LXRα expression at both mRNA and protein levels. A: HepG2 cells were transfected with 80 nM miR-613 mimic or mimic negative control (NC), after 24 hours total protein was subjected to Western blotting analysis and mRNA expression levels of LXRα were analyzed by real-time quantitative PCR and normalized with β-actin control. B and D, HepG2 cells were treated with TO901317 (5 μM) or GW3965 (2 μM) for 24 hours. Western blot analysis for LXRα protein level (B) and Real-time PCR analysis for LXRα mRNA level (D). C and E, 12 hours after transfected with 80 nM miR-613 mimic or NC, HepG2 cells were treated with TO901317 (5 μM) or GW3965 (2 μM) for 24 hours. Western blot analysis for LXRα protein level (C) and Real-time PCR analysis for LXRα mRNA level (E). The relative level of LXRα expression determined using the 2-△△CT method. *, P < 0.05 (n = 3 for each group).