Figure 3
From: MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
MiR-613 suppresses LXRα-induced lipogenic genes. A and B: HepG2 cells were treated with TO901317 (5 μM) or GW3965 (2 μM) for 24 hours. Real-time PCR analysis for SREBP-1c, FAS, ChREBP and ACC mRNA level. C and D, after 12 hours transfected with 80 nM miR-613 mimic or NC, HepG2 cells were treated with TO901317 (5 μM) or GW3965 (2 μM) for 24 hours. Real-time PCR analysis for SREBP-1c, FAS, ChREBP and ACC mRNA level. The relative level of lipogenic gene expression determined using the 2-△△CT method. *, P < 0.05 (n = 3 for each group).