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Table 1 Oligo-nucleotide primer pairs for detecting of genes promoter methylation and mRNA expression

From: Betaine supplement alleviates hepatic triglyceride accumulation of apolipoprotein E deficient mice via reducing methylation of peroxisomal proliferator-activated receptor alpha promoter

Gene symbol

Primer sequence(5′-3′)

Anneal temperature

Amplicon size

PPARa M

F: TTTTTACG AATTTTCG GGATTC

55

164

 

R: AAAAAATACG CCTTAACGCG

  

PPARa U

F: TTTTTTTATGAATTTTTGGGATTTG

55

169

 

R: TAAAAAAAATACACCTTAACACACA

  

PPARa

F: GGGCAAGAGAATCCACGAAG

60

91

 

R: GTTGTTGCTGGTCTTTCCCG

  

CYP2E1

F: TGCAGTCCGAGACAGGATGA

60

82

 

R: GGAAGGGACGAGGTTGATGA

  

CPT1

F: AGCACACCAGGCAGTAGCTT

60

144

 

R: AGGATGCCATTCTTGATTCG

  

UCP2

F: TCTCCTGAAAGCCAACCTCAT

60

272

 

R: GCTGCTCATAGGTGACAAACAT

  

ACOX

F: AGGTTCAGTCGGGGAAGCTGG

60

433

 

R: ATCTGAGCCCCTGTGATGATG

  

FAS

F: GCTGCGGAAACTTCAGGAAAT

60

84

 

R: AGAGACGTGTCACTCCTGGACTT

  

ACC

F: CACTGTGAACATGTGGAGG

60

155

 

R: AGGCTGATGGTGATGACC

  

β-Actin

F: GGGTCAGAAGGACTCCTATG

60

90

 

R: GTAACAATGCCATGTTCAAT

  
  1. Sequences with bold font mean CpG site in the primers.