Figure 1

Determination of optimal FA concentrations. Figure 1A-C illustrates the mean % live cells ± SEM of EHEB, JVM-2 and MEC-2 following 72 hour treatment with vehicle, or increasing concentrations of AA, EPA or DHA. Percent (%) live cells was determined after 72 hour treatments by trypan blue exclusion assay. Figure 1A illustrates the mean % live cells ± SEM of EHEB following treatment with vehicle, or increasing concentrations of AA, EPA or DHA. Significant reductions in % live cells were observed at 55 μM AA, 75 μM and 100 μM EPA, and 100 μM DHA as compared to vehicle. Although not statistically significant, concentrations of AA 35 μM and AA 45μM indicated viabilities of 57% and 54%. FA concentrations of AA 25 μM, EPA 50 μM and DHA 75 μM were chosen for the remainder of the study. Figure 1B illustrates the mean% live cells ± SEM of JVM-2 following treatment with vehicle, or increasing concentrations of AA, EPA or DHA. Significant reductions in % live cells were observed at 45 μM and 55 μM AA, and 75 μM and 100 μM EPA as compared to vehicle. Although not statistically significant, concentrations of DHA 75 μM and DHA 100 μM indicated cell viabilities of 65% and 55%. FA concentrations of AA 35 μM, EPA 50 μM and DHA 50 μM were chosen for the remainder of the study. Figure 1C illustrates the mean % live cells ± SEM of MEC-2 following treatment with vehicle, or increasing concentrations of AA, EPA or DHA. Significant reductions in % live cells were observed at 45 μM AA, 75 μM and 100 μM EPA and 75 μM and 100 μM DHA as compared to vehicle. FA concentrations of AA 25 μM, EPA 50 μM and DHA 50 μM were chosen for the remainder of the study. Statistical significant was determined by Multiple Comparison Test using Tukey’s correction. Abbreviations: AA- arachidonic acid, EPA- eicosapentaenoic acid, DHA- docosahexaenoic acid, α = 0.05, * <0.05, ** < 0.01, *** < 0.001.