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Figure 3 | Lipids in Health and Disease

Figure 3

From: Maternal supplementation of diabetic mice with thymoquinone protects their offspring from abnormal obesity and diabetes by modulating their lipid profile and free radical production and restoring lymphocyte proliferation via PI3K/AKT signaling

Figure 3

TQ supplementation restores lymphocyte proliferation upon antigen stimulation. PBMCs were isolated from the blood of offspring (8 weeks old) born to CD, DD, and DD + TQ, and their proliferative capacity was assessed in response to SEB following 6 days of stimulation using CFSE dilution assays and flow cytometry analysis. The plots were gated on unstimulated (A) or SEB-stimulated lymphocytes (B) of offspring born to CD, SEB-stimulated lymphocytes of offspring born to DD (C), and SEB-stimulated lymphocytes of offspring born to DD (D) and were then gated on viable cells to exclude dead cells. The numbers on the left side represent the percentage of CFSE-lo (proliferating cells) within the cell population. One representative experiment is shown. (E) The data obtained for offspring (n = 10) born to CD (black bars), DD (gray bars), and DD + TQ (hatched bars) are expressed as the mean percentages of proliferating lymphocytes ± SEM. *P < 0.05 for diabetic vs. control; #P < 0.05 for diabetic + TQ vs. diabetic; +P < 0.05 for diabetic + TQ vs. control.

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