Skip to main content
Figure 3 | Lipids in Health and Disease

Figure 3

From: C-reactive protein/oxidised low-density lipoprotein/β2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway

Figure 3

Histological quantitation of the AS lesion in aortas. After induction of diabetes and treatment with 20 μg oxLDL, 20 μg β2GPI, 40 μg oxLDL/β2GPI complex, 44 μg CRP/oxLDL/β2GPI complex and PBS (as described in the Methods section), aortas were removed, longitudinally cut open,stained with Sudan IV, or paraffin-embedded, sectioned, deparaffinated and then stained with haematoxylin and eosin. The quantitation of the AS lesion and intima thickness were measured using Image Pro-Plus 6.0. The percentage of the plaque area to the total aorta area and the ratios of intima/media thickness of lesions at the aortic roots were calculated. Results are shown as means ± SEM. A: Representative images of Sudan IV stain(4×). B: Percentage of plaque area to total aorta area (n=4 mice per group). *P < 0.05, compared with the CRP/oxLDL/β2GPI group, †P < 0.05, compared with the PBS group. C: Representative H&E stain at the root of the aortas, 200× magnification. D: The ratios of intima/media thickness of lesions at the aortic roots (n=4). *P < 0.05, compared with the CRP/oxLDL/β2GPI group, †P < 0.05, compared with the PBS group.

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X