Effect of CRP/oxLDL/β2-GPI on infiltration of inflammatory cells in the aortas. Aortas were removed, paraffin-embedded, deparaffinated, underwent antigen retrieval, blocked, and then incubated with primary antibodies (Mac 1:200 or α- SMA 1:100 or CD3 1:100) at 4°C overnight. Biotinylated secondary antibody (1:200) was added, followed by HRP-labeled streptavidin incubation. Infiltrations of macrophages, SMCs and T cells in the aortas were quantified as the percentage of the luminal surface covered by positive cells to the plaque area using Image Pro-Plus 6.0. Results are shown as means ± SEM. A: Infiltration of SMCs in the aortas, 400× magnification. B: Infiltration of macrophages in the aortas, 400× magnification. C: Infiltration of T cells in aortas, 400× magnification. D: Positive expressions of SMA, MAC and CD3 (n=4), expressed as the percentage to plaque area. *P <0.05, compared with the CRP/oxLDL/β2GPI group, †P < 0.05, compared with the PBS group.