Time course of triglyceride emulsion digestion measured by a PPARγ FP assay. A: Porcine pancreatic lipase digestion of 1.5 mg/mL triolein. Lipase concentration: 30 μg/mL (●), (□), ▲ 3.3 μg/mL, no enzyme (○). B: Candida rugosa lipase (10 μg/mL) digestion of grape seed oil emulsion. Substrate concentration: 1.5 mg/mL (●), 0. 15 mg/mL (□), (▲) 0.015 mg/mL, no substrate (○). C: Digestion of three different substrates each at 0.15 mg/ml with 10 μg/ml Candida rugosa lipase: grape seed oil (●), olive oil (□) and triolein (▲). D: Data from Figure 1A plotted as increasing mP change and fitted for initial rate using MonoMolecular Curve fit to determine initial velocity. E: Initial velocities of lipase digestion reactions from Figure 1A/1D.