Figure 3
From: PPARγ as a sensor of lipase activity and a target for the lipase inhibitor orlistat
The molecular weights of PPARγ and PPARγ conjugates were measured by ESI-MS. The software package DataAnalysis (Bruker, Bremen, Germany) was used to average sample spectra and molecular weights calculated following charge deconvolution. The displayed mass spectra are of the molecular ion containing 40 positive charges and clearly show that the molecular weight of PPARγ increased when IAF is bound, indicating covalent binding. Whereas, there is no change in the molecular weight of PPARγ when Orlistat is bound, suggesting that the binding of PPARγ to Orlistat is by non-covalent mechanisms.