Table 2 Primers for real-time polymerase chain reaction
Gene | Primer sequences |
|---|---|
E-cadherin | 5′-AAATCTGAAAGCGGCTGATACTG-3′-sense |
5′-CGGAACCGCTTCCTTCATAG-3′-antisense | |
α-SMA | 5′-GACAATGGCTCTGGGCTCTGTAA-3′-sense |
5′-ATGCCATGTTCTATCGGGTACTTCA-3′-antisense | |
angiotensinogen | 5′-GATGTTGCTGCTGAGAAGATTG-3′-sense |
5′-GGAAGTGGACGTAGGTGTTGA-3′-antisense | |
renin | 5′-GAGGCTGACACTTGGCAACA-3′-sense |
5′-CGCCATAGTACTGGGTGTCCAT-3′-antisense | |
ACE | 5′-CACTATCAAGCGGATCATAAAGAAG-3′-sense |
5′-CACGCTGTAGGTGGTTTCCATA-3′-antisense | |
AT1 | 5′-ACCTGGCTATTGTTCACCCAAT-3′-sense |
5′-TGCAGGTGACTTTGGCTACAAG-3′-antisense | |
AT2 | 5′-CCACCCTTGCCACTACTAGCA-3′-sense |
5′-ATTGTTGCCAGAGATGTTCACAA-3′-antisense | |
β-actin | 5′-AAAGACCTGTACGCCAACAC-3′-sense |
5′-GTCATACTCCTGCTTGCTGAT-3′-antisense |