Figure 1

GH and insulin- induced STAT5 and MAPK activation in differentiated 3T3-F442A adipocytes. STAT5, MAPK and AKT activations were analyzed by Western blot using specific antibodies against phosphorylated forms of STAT5, MAPK and AKT (A-C). Densitometric analysis of the results from Western Blots (D-K). A, D and F, STAT5 and MAPK activation induced by GH at various concentrations for 10 min (n = 4). B, E and G, STAT5 and MAPK activation induced by a single dose of GH (125 ng/ml) for indicated times (n = 4). C, H to K, STAT5 MAPK and AKT activation induced by IGF-I (100 ng/ml) or insulin (200 nM) (n = 3).