Figure 3

Insulin pretreatment on GH induced STAT5 activation in vitro (A, B) and in vivo (C,D). A, B, pretreatment with 10 nM or 100 nM insulin (insulin was added 20 min prior to GH) on GH- induced STAT5 activation in differentiated 3T3-F442A cells (n = 4). C, D, visceral adipose tissues around the kidneys were extracted from C57/BL6 mice with GH (50 μg/kg. BW), GH (50 μg/kg. BW) + insulin (2 μM/kg. BW injected prior 20 min to GH) or saline injection (n = 5). ^*p < 0.05, GH alone vs. control; GH + insulin vs. GH alone.